Construction of a recombinant Bacillus velezensis strain as an integrated control agent against plant diseases and insect pests.
Identifieur interne : 001B49 ( Main/Exploration ); précédent : 001B48; suivant : 001B50Construction of a recombinant Bacillus velezensis strain as an integrated control agent against plant diseases and insect pests.
Auteurs : Jong Yul Roh [Corée du Sud] ; Qin Liu ; Jae Young Choi ; Yong Wang ; Hee Jin Shim ; Hong Guang Xu ; Gyung Ja Choi ; Jin-Cheol Kim ; Yeon Ho JeSource :
- Journal of microbiology and biotechnology [ 1017-7825 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Antibiose (MeSH), Bacillus (composition chimique), Bacillus (génétique), Bacillus (physiologie), Champignons (physiologie), Clonage moléculaire (MeSH), Endotoxines (génétique), Endotoxines (métabolisme), Génie génétique (MeSH), Hémolysines (génétique), Hémolysines (métabolisme), Lutte biologique contre les nuisibles (méthodes), Maladies des plantes (microbiologie), Maladies des plantes (parasitologie), Papillons de nuit (microbiologie), Papillons de nuit (physiologie), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme).
- MESH :
- composition chimique : Bacillus.
- génétique : Bacillus, Endotoxines, Hémolysines, Protéines bactériennes.
- microbiologie : Maladies des plantes, Papillons de nuit.
- métabolisme : Endotoxines, Hémolysines, Protéines bactériennes.
- méthodes : Lutte biologique contre les nuisibles.
- parasitologie : Maladies des plantes.
- physiologie : Bacillus, Champignons, Papillons de nuit.
- Animaux, Antibiose, Clonage moléculaire, Génie génétique.
English descriptors
- KwdEn :
- Animals (MeSH), Antibiosis (MeSH), Bacillus (chemistry), Bacillus (genetics), Bacillus (physiology), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Cloning, Molecular (MeSH), Endotoxins (genetics), Endotoxins (metabolism), Fungi (physiology), Genetic Engineering (MeSH), Hemolysin Proteins (genetics), Hemolysin Proteins (metabolism), Moths (microbiology), Moths (physiology), Pest Control, Biological (methods), Plant Diseases (microbiology), Plant Diseases (parasitology).
- MESH :
- chemical , genetics : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- chemistry : Bacillus.
- genetics : Bacillus.
- chemical , metabolism : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- methods : Pest Control, Biological.
- microbiology : Moths, Plant Diseases.
- parasitology : Plant Diseases.
- physiology : Bacillus, Fungi, Moths.
- Animals, Antibiosis, Cloning, Molecular, Genetic Engineering.
Abstract
To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans), and wheat leaf rust (Puccinia recondita), by electroporation. The recombinant B. velezensis strain was confirmed by PCR using cry1Ac-specific primers. Additionally, the recombinant strain produced a protein approximately 130 kDa in size and parasporal inclusion bodies similar to B. thuringiensis. The in vivo antifungal activity assay demonstrated that the activity of the recombinant B. velezensis strain was maintained at the same level as that of wild-type B. velezensis. Furthermore, it exhibited high insecticidal activity against a lepidopteran pest, Plutella xylostella, although its activity was lower than that of a recombinant B. thuringiensis strain, whereas wild-type B. velezensis strain did not show any insecticidal activity. These results suggest that this recombinant B. velezensis strain can be used to control harmful insect pests and fungal diseases simultaneously in one crop.
DOI: 10.4014/jmb.0902.065
PubMed: 19884784
Affiliations:
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wicri:level="4"><nlm:affiliation>Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Liu, Qin" sort="Liu, Qin" uniqKey="Liu Q" first="Qin" last="Liu">Qin Liu</name></author><author><name sortKey="Choi, Jae Young" sort="Choi, Jae Young" uniqKey="Choi J" first="Jae Young" last="Choi">Jae Young Choi</name></author><author><name sortKey="Wang, Yong" sort="Wang, Yong" uniqKey="Wang Y" first="Yong" last="Wang">Yong Wang</name></author><author><name sortKey="Shim, Hee Jin" sort="Shim, Hee Jin" uniqKey="Shim H" first="Hee Jin" last="Shim">Hee Jin Shim</name></author><author><name sortKey="Xu, Hong Guang" sort="Xu, Hong Guang" uniqKey="Xu H" first="Hong Guang" last="Xu">Hong Guang Xu</name></author><author><name sortKey="Choi, Gyung Ja" sort="Choi, Gyung Ja" uniqKey="Choi G" first="Gyung Ja" last="Choi">Gyung Ja Choi</name></author><author><name sortKey="Kim, Jin Cheol" sort="Kim, Jin Cheol" uniqKey="Kim J" first="Jin-Cheol" last="Kim">Jin-Cheol Kim</name></author><author><name sortKey="Je, Yeon Ho" sort="Je, Yeon Ho" uniqKey="Je Y" first="Yeon Ho" last="Je">Yeon Ho Je</name></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="ISSN">1017-7825</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antibiosis (MeSH)</term><term>Bacillus (chemistry)</term><term>Bacillus (genetics)</term><term>Bacillus (physiology)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Endotoxins (genetics)</term><term>Endotoxins (metabolism)</term><term>Fungi (physiology)</term><term>Genetic Engineering (MeSH)</term><term>Hemolysin Proteins (genetics)</term><term>Hemolysin Proteins (metabolism)</term><term>Moths (microbiology)</term><term>Moths (physiology)</term><term>Pest Control, Biological (methods)</term><term>Plant Diseases (microbiology)</term><term>Plant Diseases (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Antibiose (MeSH)</term><term>Bacillus (composition chimique)</term><term>Bacillus (génétique)</term><term>Bacillus (physiologie)</term><term>Champignons (physiologie)</term><term>Clonage moléculaire (MeSH)</term><term>Endotoxines (génétique)</term><term>Endotoxines (métabolisme)</term><term>Génie génétique (MeSH)</term><term>Hémolysines (génétique)</term><term>Hémolysines (métabolisme)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Maladies des plantes (microbiologie)</term><term>Maladies des plantes (parasitologie)</term><term>Papillons de nuit (microbiologie)</term><term>Papillons de nuit (physiologie)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Endotoxins</term><term>Hemolysin Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bacillus</term><term>Endotoxines</term><term>Hémolysines</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>Endotoxins</term><term>Hemolysin Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Papillons de nuit</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Moths</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Endotoxines</term><term>Hémolysines</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Bacillus</term><term>Champignons</term><term>Papillons de nuit</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Bacillus</term><term>Fungi</term><term>Moths</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibiosis</term><term>Cloning, Molecular</term><term>Genetic Engineering</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antibiose</term><term>Clonage moléculaire</term><term>Génie génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans), and wheat leaf rust (Puccinia recondita), by electroporation. The recombinant B. velezensis strain was confirmed by PCR using cry1Ac-specific primers. Additionally, the recombinant strain produced a protein approximately 130 kDa in size and parasporal inclusion bodies similar to B. thuringiensis. The in vivo antifungal activity assay demonstrated that the activity of the recombinant B. velezensis strain was maintained at the same level as that of wild-type B. velezensis. Furthermore, it exhibited high insecticidal activity against a lepidopteran pest, Plutella xylostella, although its activity was lower than that of a recombinant B. thuringiensis strain, whereas wild-type B. velezensis strain did not show any insecticidal activity. These results suggest that this recombinant B. velezensis strain can be used to control harmful insect pests and fungal diseases simultaneously in one crop.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19884784</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1017-7825</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>10</Issue><PubDate><Year>2009</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of microbiology and biotechnology</Title><ISOAbbreviation>J Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Construction of a recombinant Bacillus velezensis strain as an integrated control agent against plant diseases and insect pests.</ArticleTitle><Pagination><MedlinePgn>1223-9</MedlinePgn></Pagination><Abstract><AbstractText>To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans), and wheat leaf rust (Puccinia recondita), by electroporation. The recombinant B. velezensis strain was confirmed by PCR using cry1Ac-specific primers. Additionally, the recombinant strain produced a protein approximately 130 kDa in size and parasporal inclusion bodies similar to B. thuringiensis. The in vivo antifungal activity assay demonstrated that the activity of the recombinant B. velezensis strain was maintained at the same level as that of wild-type B. velezensis. Furthermore, it exhibited high insecticidal activity against a lepidopteran pest, Plutella xylostella, although its activity was lower than that of a recombinant B. thuringiensis strain, whereas wild-type B. velezensis strain did not show any insecticidal activity. These results suggest that this recombinant B. velezensis strain can be used to control harmful insect pests and fungal diseases simultaneously in one crop.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Roh</LastName><ForeName>Jong Yul</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Qin</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Jae Young</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yong</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Shim</LastName><ForeName>Hee Jin</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Hong Guang</ForeName><Initials>HG</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Gyung Ja</ForeName><Initials>GJ</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jin-Cheol</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Je</LastName><ForeName>Yeon Ho</ForeName><Initials>YH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>J Microbiol Biotechnol</MedlineTA><NlmUniqueID>9431852</NlmUniqueID><ISSNLinking>1017-7825</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004731">Endotoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C030956">insecticidal crystal protein, Bacillus Thuringiensis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000898" MajorTopicYN="N">Antibiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001407" MajorTopicYN="N">Bacillus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004731" MajorTopicYN="N">Endotoxins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="Y">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>11</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>11</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19884784</ArticleId><ArticleId IdType="pii">JMB019-10-23</ArticleId><ArticleId IdType="doi">10.4014/jmb.0902.065</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><noCountry><name sortKey="Choi, Gyung Ja" sort="Choi, Gyung Ja" uniqKey="Choi G" first="Gyung Ja" last="Choi">Gyung Ja Choi</name><name sortKey="Choi, Jae Young" sort="Choi, Jae Young" uniqKey="Choi J" first="Jae Young" last="Choi">Jae Young Choi</name><name sortKey="Je, Yeon Ho" sort="Je, Yeon Ho" uniqKey="Je Y" first="Yeon Ho" last="Je">Yeon Ho Je</name><name sortKey="Kim, Jin Cheol" sort="Kim, Jin Cheol" uniqKey="Kim J" first="Jin-Cheol" last="Kim">Jin-Cheol Kim</name><name sortKey="Liu, Qin" sort="Liu, Qin" uniqKey="Liu Q" first="Qin" last="Liu">Qin Liu</name><name sortKey="Shim, Hee Jin" sort="Shim, Hee Jin" uniqKey="Shim H" first="Hee Jin" last="Shim">Hee Jin Shim</name><name sortKey="Wang, Yong" sort="Wang, Yong" uniqKey="Wang Y" first="Yong" last="Wang">Yong Wang</name><name sortKey="Xu, Hong Guang" sort="Xu, Hong Guang" uniqKey="Xu H" first="Hong Guang" last="Xu">Hong Guang Xu</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Roh, Jong Yul" sort="Roh, Jong Yul" uniqKey="Roh J" first="Jong Yul" last="Roh">Jong Yul 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